FastQCFastQC Report
Sun 14 Apr 2019
SRR6575897_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575897_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1010051
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA27080.2681052738921104No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC24630.24384907296760264No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA21880.21662272499111432No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC21540.21325655833220303No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT19730.19533667111858707No Hit
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC14720.14573521535051198No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT11160.11048947033367622No Hit
GTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGG10500.10395514681931903No Hit
GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC10370.10266808309679412No Hit
GTTTGGGACTGACCCTAGTCTGTGTCCATGCAGAAGAAGCTAGTTCTACG10240.10138101937426922No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTAACG2650.035.484742
TAGGTAT5150.034.6803445
AGGTATA5250.033.1228686
CTTAGGT5600.032.732833
CTAACGC2900.032.414483
AAGGGTA3550.031.7753185
AACCGTG2250.029.2436127
CGCCTAC3250.028.9222537
ACCTAAG9450.028.857171
TTAGGTA6050.028.7444084
TAAGGGT1503.6961865E-828.2005944
GTGCAAG4200.027.9864771
ACGCCTA3400.027.6462756
GTCTAAC3600.027.426751
TGAACCG2400.027.4172465
CTAAGAC11750.026.8005623
CATGGGG33200.026.7565884
GTACATG71850.026.6333121
TAAGACA13150.026.4492264
TACATGG73050.026.2601662