FastQCFastQC Report
Sun 14 Apr 2019
SRR6575897_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575897_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1010051
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA28780.2849361071866668No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC25030.24780926903691003No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC19570.19375259269086412No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA19570.19375259269086412No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT18150.17969389664482288No Hit
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC14830.14682426926957154No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT11190.11078648503887428No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGTCG550.002715723634.1847155
TAGGTAT6600.031.3359895
GTACTAG3800.031.0081121
AAGGGTA2300.030.6547725
TTAGGTA6850.030.1923434
TAGTACT5450.029.3235874
CTTAGGT7200.028.7246573
ACCTAAG10200.028.6490611
GTGCAAG3900.027.7959881
AGGTATA7300.027.6872796
GTATCAA41900.027.446991
GTACCGT700.00883187126.8594196
CATGGGG33050.026.4530754
GGGTACC3050.026.1950537
CTAAGAC11550.026.0454983
ATGGGGG16600.025.7672445
GTACTAT1301.0641941E-525.3789481
TCTTAGG8550.025.2886942
CAAGGGT4100.025.2216514
GGTAATC2800.025.1769688
Produced by FastQC (version 0.11.2)