FastQCFastQC Report
Sun 14 Apr 2019
SRR6575898_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575898_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences927703
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA28070.3025752854092312No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC22370.24113320750283224No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA21600.2328331373295117No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT19980.2153706520297983No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC19600.21127451350270507No Hit
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC13270.14304146909086204No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT10100.10887105032537353No Hit
GTTTGGGACTGACCCTAGTCTGTGTCCATGCAGAAGAAGCTAGTTCTACG9410.10143332510512523No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCTAAG10350.031.843011
AGGTATA5800.031.598976
GTACTAG2750.030.8174131
CTTAGGT6800.029.7163813
TAGGTAT6350.029.6021065
TAGTACT6200.029.5603284
TTAGGTA6600.028.4808164
CCTAAGA11900.028.4329472
GGGCTAT3650.028.3247536
CATGGGG29550.028.1482984
CTAAGAC12200.028.1189693
TCTTAGG8350.027.5769312
TAAGACA13250.027.3093384
GTACTGT8100.027.2677426
GTCTATA1606.797018E-826.4837151
CTAGTAC7150.026.2899843
GGTATAG7000.026.1805957
CTGTGCG8100.026.12299
AGGGCTA4400.025.6327325
GTATCAA40500.025.342991
Produced by FastQC (version 0.11.2)