FastQCFastQC Report
Sun 14 Apr 2019
SRR6575900_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575900_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences947114
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA31400.3315334796022443No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT27330.28856082794679416No Hit
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC20210.21338508352743177No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT16010.16903984103286404No Hit
GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC15200.16048754426605455No Hit
GACCATAATGTGATCCCTTCCGGCGGTCGGTATAAAATCAGGCAGTTTTT13540.14296061508963018No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA13160.1389484264829788No Hit
GTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGG12580.13282455966230042No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA11550.1219494168600612No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT11470.12110474557445038No Hit
CAATTGATATTAGATATTTTTGCAAAGCGGGCGAGAACGAGAGAAGGCAA10940.11550879830727874No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA10910.11519204657517469No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA10810.11413620746816118No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG10600.11191894534343279No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG10010.10568949461205303No Hit
GTTAATAATTCTGGCAATTCGTCTCCACACTAGAAGTCGACGAACAACGA9990.10547832679065033No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA9660.10199405773750572No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAG6550.043.7902831
TAGGCAT8600.035.512645
CTAGGCA9700.031.9698264
TTAACAC804.7886264E-429.3662223
TACTAGG10750.028.431132
CAATGCG4800.028.38734818-19
GTATAAG4150.028.325731
GGACCGT856.827111E-427.63886
ACTAGGC11350.026.9082583
AGTCCTA5200.026.65549528-29
CATGGGG23700.026.3676474
CTAACTG2500.026.3121384
GAGTTGC10200.026.25685794
ACCTAAG2156.548362E-1126.2441161
CGACGAG5250.025.95414724-25
ATCGTCA5350.025.46902510-11
GTAGGAC5550.024.5512223
CATCGTC11500.024.5144129
TAGTACT7300.024.4584454
ACGCAAG1351.4604657E-524.363098
Produced by FastQC (version 0.11.2)