FastQCFastQC Report
Sun 14 Apr 2019
SRR6575900_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575900_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences947114
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA28910.30524308583760773No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT28080.29647962124939553No Hit
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC19660.20757796843885742No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT15120.15964287298044375No Hit
GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC14500.15309667051695994No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA12370.13060729753757205No Hit
GACCATAATGTGATCCCTTCCGGCGGTCGGTATAAAATCAGGCAGTTTTT11160.11783164434270847No Hit
GTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGG11020.11635346959288956No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA10650.11244686489693953No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT10600.11191894534343279No Hit
CAATTGATATTAGATATTTTTGCAAAGCGGGCGAGAACGAGAGAAGGCAA10460.11044077059361387No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG10090.10653416589766385No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC9850.1040001520408314No Hit
GTTAATAATTCTGGCAATTCGTCTCCACACTAGAAGTCGACGAACAACGA9730.1027331451124152No Hit
GTATAAGGTGATCGCAGGTTGTGCAATCATTGCTCAAAAGGGTGTACACC9700.10241639338031112No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT9520.10051588298768681No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTTAC500.001686295337.6704641
TCTACGT1001.4123161E-632.8955272
GTATATA1605.820766E-1132.3730551
GTACTAG4700.031.0580941
ACCTAAG2500.030.1363721
TAGTACT8200.027.5070044
GTCTAAT1057.326256E-526.9074751
AGTACCG1755.5624696E-926.8520745
TACTGTG9300.026.7798927
CTGTGCG8900.026.417649
CGATAGT909.4129925E-426.1600441
TATTAAG1807.50515E-926.1075612
GTACTGT9250.025.9086236
CATGGGG24150.025.6845914
CATAAGT1101.0194576E-425.6315274
GTATAAG4700.025.046851
CTAGTAC9400.024.9952833
ATGGGGG14250.024.4024125
TATATAA1951.7582352E-824.0992892
ACTGTGC10650.023.8428548
Produced by FastQC (version 0.11.2)