FastQCFastQC Report
Sun 14 Apr 2019
SRR6575902_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575902_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1306138
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA35410.27110458466103887No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC33590.25717037556521594No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC29340.2246317004788162No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG16530.12655630568898538No Hit
ACCCTAGTCTGTGTCCATGCAGAAGAAGCTAGTTCTACGGGAAGGAACTT15020.11499550583475865No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA14800.11331115088910973No Hit
GTTTGGGACTGACCCTAGTCTGTGTCCATGCAGAAGAAGCTAGTTCTACG14740.11285178135847819No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC14330.10971275623249611No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA14150.10833464764060154No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT13710.10496593774930367No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT13440.1028987748614618No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA13130.1005253656198656No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA24550.049.397441
TCAACGC29600.040.1620064
CAACGCA30150.039.4293675
ATCAACG30450.039.04093
AACGCAG31650.037.85766
GGTATCA10850.036.823531
TATAGCG550.002720558834.173065
TAGGTAT9750.033.7349435
TATCAAC35850.033.3028072
CTTAGGT9700.032.940013
AGGTATA9700.032.45566
TTAGGTA10050.032.260394
GTCTTAG11150.031.1955831
GGTATAG10200.030.8634597
TCTTAGG11850.029.7494642
ACGCAGA40250.029.6508927
GTACATG57450.028.4724921
GTATAGT11050.028.0641338
ACCTAAG11950.027.9271561
TATAGTA11150.027.8124379
Produced by FastQC (version 0.11.2)