FastQCFastQC Report
Sun 14 Apr 2019
SRR6575903_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575903_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences584049
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG11640.19929834654284143No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8590.14707670075627216No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA7390.12653047946319573No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA6820.11677102434898441No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA6720.11505883924122805No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTG6510.11146325051493966No Hit
CCTATAAGCAGTTCTTGTATTTTTATTCACACAGGTCTGATTAAGATGAA6330.10838131732097821No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT6150.10529938412701674No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA13500.057.238741
ATCAACG19950.037.926783
TCAACGC20250.037.5969854
CAACGCA20850.036.515065
AACGCAG21200.035.9122166
GTACATG34600.034.5889661
ACATGGG35200.034.179083
TACATGG35200.033.7785452
CATGGGG13800.033.7146874
GGTATCA5650.031.689481
GTACTAG1052.0233329E-631.4115031
CCTATAA4050.031.4115031
TAGTGCG600.004160952331.3308245
TATCAAC24350.031.2664872
ACGCAGA24050.031.0702727
CTATAAG3800.029.681832
CGCAGAG24950.029.572788
GTAATAC1005.3220163E-528.197743
TATAAGC3850.028.075673
TATATAC1555.0784365E-827.2881363