FastQCFastQC Report
Sun 14 Apr 2019
SRR6575904_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575904_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1116014
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA50530.4527720978410665No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC40760.36522839319220013No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC30200.2706059242984407No Hit
ATCCTAGGTAACATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAG21070.18879691473404456No Hit
GAATTTATACTGGGGATGCTGTATGGATAGGAAGGGATGATGGTGGAGTC19520.17490820007634314No Hit
ACCCTAGTCTGTGTCCATGCAGAAGAAGCTAGTTCTACGGGAAGGAACTT18550.16621655283894288No Hit
GTTTGGGACTGACCCTAGTCTGTGTCCATGCAGAAGAAGCTAGTTCTACG17590.15761451021223746No Hit
CATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAGGATGGAATGCA15130.13557177598130488No Hit
GTGTAGCCACGATCACAAGAAAGACGTGGTCCTGACAGACAGACAATCCT15060.13494454370644096No Hit
ATCCTATTCCCTACCAAAATGAAGATGCTGTTGCTGCTGTGTTTGGGACT13920.12472961808722831No Hit
TCACATAGTTGTGCAAACCTTTCCTTGATGTCTGAACTCAAATCTGGTTC13580.12168306132360347No Hit
GTGCAAGGGTAATCATTTATTGAACAGGAAGAGGAAGAAATTCATGAAAA13170.1180092722851147No Hit
GCATTGGATAGGTCAATGATATTTTCTCTAAGGATTCCATGCTCCTCACA12560.11254339103272898No Hit
ATCTATGGTTGCTGACAAAACAGAAAAGGCTGGTGAATATTCTGTGACGT12070.10815276510868144No Hit
GGCCTGGAGGCAGCGATTGGCATTGGATAGGTCAATGATATTTTCTCTAA11940.1069879051696484No Hit
GTATGGATAGGAAGGGATGATGGTGGAGTCCTGGTGAGAAGTCTCCACTC11870.10636067289478447No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11870.10636067289478447No Hit
CTGTAAGAGATGAAGAGTGCTCCGAATTATCTATGGTTGCTGACAAAACA11830.10600225445200509No Hit
GGATAGGTCAATGATATTTTCTCTAAGGATTCCATGCTCCTCACATAGTT11220.10053637319961937No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGGGTA5450.042.2551085
GTATCAA38300.041.2435841
GGTAATC5600.041.1232768
TCAACGC46100.034.2545284
ATCAACG46300.034.005053
CAACGCA46650.033.7499245
GTGCAAG6900.033.385881
AACGCAG47750.033.366146
TATCAAC48250.032.738432
AGGTATA9950.032.59166
AGGGTAA6950.032.459076
GTAATCA7100.032.435269
GTCTTAG12000.032.1253931
GTACATG78750.031.759741
TAGGTAT10100.031.6422375
CTTAGGT10400.031.1813853
TACATGG80800.030.895772
ACATGGG79800.030.6841773
GTATAGT10500.030.4368178
TCTTAGG13350.029.9332282
Produced by FastQC (version 0.11.2)