FastQCFastQC Report
Sun 14 Apr 2019
SRR6575905_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575905_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences987083
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA47310.4792910018711699No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC37600.38092034813688413No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC28760.2913635428834252No Hit
ATCCTAGGTAACATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAG20100.2036302924880684No Hit
GAATTTATACTGGGGATGCTGTATGGATAGGAAGGGATGATGGTGGAGTC16760.16979321900995153No Hit
ACCCTAGTCTGTGTCCATGCAGAAGAAGCTAGTTCTACGGGAAGGAACTT16420.16634872650020313No Hit
GTTTGGGACTGACCCTAGTCTGTGTCCATGCAGAAGAAGCTAGTTCTACG15710.1591558156710226No Hit
CATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAGGATGGAATGCA13730.13909671223189946No Hit
TCACATAGTTGTGCAAACCTTTCCTTGATGTCTGAACTCAAATCTGGTTC12800.12967501213170524No Hit
GTGTAGCCACGATCACAAGAAAGACGTGGTCCTGACAGACAGACAATCCT12280.12440696476385471No Hit
GTGCAAGGGTAATCATTTATTGAACAGGAAGAGGAAGAAATTCATGAAAA12240.12400173035094313No Hit
ATCCTATTCCCTACCAAAATGAAGATGCTGTTGCTGCTGTGTTTGGGACT11790.11944284320568788No Hit
GCATTGGATAGGTCAATGATATTTTCTCTAAGGATTCCATGCTCCTCACA11500.11650489371207894No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11220.11366825282169787No Hit
ATCTATGGTTGCTGACAAAACAGAAAAGGCTGGTGAATATTCTGTGACGT11130.11275647539264681No Hit
GGCCTGGAGGCAGCGATTGGCATTGGATAGGTCAATGATATTTTCTCTAA11050.11194600656682366No Hit
GGATAGGTCAATGATATTTTCTCTAAGGATTCCATGCTCCTCACATAGTT10890.11032506891517735No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA10390.10525963875378261No Hit
GTATGGATAGGAAGGGATGATGGTGGAGTCCTGGTGAGAAGTCTCCACTC10210.10343608389568051No Hit
CTGTAAGAGATGAAGAGTGCTCCGAATTATCTATGGTTGCTGACAAAACA10170.10303084948276892No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA37700.043.3899731
TGCGTAT450.001015040241.7681294
ATCAACG46400.034.835013
TCAACGC46500.034.6590464
CAACGCA47000.034.2903335
AACGCAG48300.033.3674056
TATCAAC49200.032.9613882
CCATACG600.004166443431.3245034
GTACTTG6300.030.5910781
CTTAGGT9100.030.463943
TAGGTAT9200.029.6220845
TTAGGTA9550.029.5204754
AGGTATA9250.029.4619646
GTACATG70350.029.1322381
AAGGGTA4850.029.063975
GGTAATC4600.028.6006348
TACATGG71600.028.3610422
ACGCAGA56950.028.2993097
ACATGGG72100.027.8922753
CGCAGAG58550.027.6062248
Produced by FastQC (version 0.11.2)