FastQCFastQC Report
Sun 14 Apr 2019
SRR6575905_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575905_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences987083
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA51210.5188013571300488No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC35820.36288741676231884No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC26670.27019004480879516No Hit
GTTTGGGACTGACCCTAGTCTGTGTCCATGCAGAAGAAGCTAGTTCTACG16230.1644238630388731No Hit
ATCCTAGGTAACATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAG15370.15571132316127417No Hit
ACCCTAGTCTGTGTCCATGCAGAAGAAGCTAGTTCTACGGGAAGGAACTT14950.1514563618257026No Hit
GAATTTATACTGGGGATGCTGTATGGATAGGAAGGGATGATGGTGGAGTC13650.1382862434060763No Hit
ATCCTATTCCCTACCAAAATGAAGATGCTGTTGCTGCTGTGTTTGGGACT11620.11772059695081365No Hit
GTGTAGCCACGATCACAAGAAAGACGTGGTCCTGACAGACAGACAATCCT11590.11741667114112998No Hit
TCACATAGTTGTGCAAACCTTTCCTTGATGTCTGAACTCAAATCTGGTTC11410.11559311628302787No Hit
CATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAGGATGGAATGCA11250.11397217863138155No Hit
GCATTGGATAGGTCAATGATATTTTCTCTAAGGATTCCATGCTCCTCACA10980.1112368463442284No Hit
ATCTATGGTTGCTGACAAAACAGAAAAGGCTGGTGAATATTCTGTGACGT10620.1075897366280242No Hit
CTGTAAGAGATGAAGAGTGCTCCGAATTATCTATGGTTGCTGACAAAACA10590.1072858108183405No Hit
GGCCTGGAGGCAGCGATTGGCATTGGATAGGTCAATGATATTTTCTCTAA10090.10222038065694576No Hit
GGATAGGTCAATGATATTTTCTCTAAGGATTCCATGCTCCTCACATAGTT10070.10201776345048998No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9880.10009289998915998No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA35350.041.8096851
AGGTATA8200.036.1042866
GTATAGT8900.034.343268
CTTAGGT9050.033.7517933
TAGGTAT8650.033.6827625
TCAACGC43850.033.543384
CAACGCA43850.033.4362145
GTCTTAG9600.033.340671
TATCAAC44350.033.2745482
ATCAACG44400.033.2337043
AACGCAG44750.032.658746
ACCTAAG17850.032.1705361
GGTATAG9350.031.6636547
ACCAGAT18750.030.8492194
TTAGGTA9950.029.7542864
CTAAGAC19800.029.6672233
TAAGACA20500.029.3418964
TATAGTA10500.029.1100039
AAGGGTA4200.029.0908325
ATGGGAG10700.028.9862635