FastQCFastQC Report
Sun 14 Apr 2019
SRR6575909_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575909_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1309351
Sequences flagged as poor quality0
Sequence length100
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT21530.164432608215826No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG19350.1477831383639681No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA18500.14129137259604185No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA17820.13609795998170085No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA16950.12945344678394105No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA16540.1263221244723531No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC15150.11570617809892075No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT14650.11188749235308179No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCG14300.1092144123309945No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT14230.10867979632657705No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT13710.10470836315090452No Hit
GAATAATGGAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAG13540.10341000999731928No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA20700.052.904911
ATCAACG26350.040.4768183
TCAACGC26400.040.4001584
CAACGCA26500.040.2477045
AACGCAG28450.037.654226
TAGGTAT3950.033.3059655
TTAGGTA4350.031.3234674
GTCTTAG5650.030.7796481
GGTATCA8050.030.3611261
ACGCAGA35650.030.0494447
GTACATG53700.029.9338251
AGGTATA4100.029.7954946
TACATGG52900.029.7645652
TATCAAC37050.029.0507722
ACATGGG54750.028.7489363
CGCAGAG38550.027.7889148
GTACGGA856.815447E-427.6478581
CTTAGGT4450.027.4520283
GGTATAG4700.025.9918147
GCAGAGT43300.024.95759
Produced by FastQC (version 0.11.2)