FastQCFastQC Report
Sun 14 Apr 2019
SRR6575909_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575909_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1309351
Sequences flagged as poor quality0
Sequence length100
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG17950.13709081827561898No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT16900.12907157820935716No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA16600.12678036676185378No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT16200.1237254181651826No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA15890.12135783300276244No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC15140.115629804384004No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA14420.11013089690999588No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA14190.10837430146690993No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCG14020.10707594831332469No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT13990.10684682716857435No Hit
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA13660.10432649457632064No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT13330.10180616198406693No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA18950.056.1015321
TCAACGC23600.044.6012344
ATCAACG24000.043.8578833
CAACGCA24800.042.4447335
AACGCAG25750.041.0613066
GGTATCA7400.036.8698841
TAGGTAT4050.035.9694755
AGGTATA3950.033.3110586
CTTAGGT4100.033.2372443
TATCAAC33000.032.4687042
ACGCAGA32850.032.043517
TTAGGTA4550.029.9500454
GTCTTAG6200.029.5902331
TACATGG47400.029.5448632
CGCAGAG35850.029.3833668
GTACATG48900.029.244231
ACATGGG50500.027.6360473
TAACTCG700.00884509626.8517654
GCAGAGT39700.026.7707569
GGTATAG4950.025.6322087
Produced by FastQC (version 0.11.2)