FastQCFastQC Report
Sun 14 Apr 2019
SRR6575910_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575910_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1862548
Sequences flagged as poor quality0
Sequence length100
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG22220.11929893887298476No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT21740.11672182408184917No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT20910.11226556308884389No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC20800.11167497428254199No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA20790.11162128439105999No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT19840.10652074470027081No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA19710.10582277611100492No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCG19030.1021718634902295No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA27250.049.299951
ATCAACG33850.038.7466553
TCAACGC34800.037.8209534
CAACGCA35900.037.0539055
GGTATCA12500.036.2017331
AACGCAG37350.035.6154026
GTCTTAG9000.031.9488661
GTACATG68350.029.379151
TATCAAC46600.028.5488872
TACATGG69200.028.2602422
ACGCAGA46600.028.1378147
ACATGGG70650.027.2144643
AGGTATA6400.027.1746396
ACCTAAG8450.026.2185881
CTAAGAC12550.025.8460433
CTTAGGT6750.025.768353
CGCAGAG51050.025.5929958
TAGGTAT6850.025.3894425
TTAGGTA7200.024.1558824
TAAGACA13450.023.415654
Produced by FastQC (version 0.11.2)