FastQCFastQC Report
Sun 14 Apr 2019
SRR6575911_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575911_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1662126
Sequences flagged as poor quality0
Sequence length100
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG25710.15468141404442262No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT22720.13669240478760336No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA22400.13476715964974978No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA22130.13314273406468582No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC21720.13067601373181095No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA21640.13019470244734754No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA20780.12502060613936608No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT20030.1205083128475218No Hit
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA18440.11094225106881186No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT17870.1075129081670102No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT17860.10745274425645228No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA27400.051.130441
GGTATCA11800.043.825261
TCAACGC35050.038.7414054
ATCAACG35700.038.299253
CAACGCA36100.037.744735
AACGCAG37650.036.3156246
ACGCAGA45050.030.141767
TATCAAC48400.028.557212
CGCAGAG49050.027.6837168
GTACATG65750.027.5997641
TACATGG66800.026.9548022
ACATGGG68150.025.7852443
TAGGTAT5200.025.299985
GCAGAGT54600.024.9557659
CTTAGGT5650.024.9481493
ACCTAAG6900.024.5282861
AGGTATA5300.023.9361026
TTAGGTA6100.023.107714
CTAAGAC9700.022.7662563
GTCTTAG8050.022.192261