FastQCFastQC Report
Sun 14 Apr 2019
SRR6575911_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575911_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1662126
Sequences flagged as poor quality0
Sequence length100
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG22950.1380761747304356No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT21350.12844994904116774No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC19860.11948552636803708No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA19700.1185229037991103No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA19340.11635700301902503No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT19140.11515372480786655No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT18500.11130323453215941No Hit
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA18430.11088208715825394No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA18040.10853569464649492No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA17750.1067909412403151No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCG16680.10035340281061725No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA24300.054.820221
TCAACGC30650.042.0108534
ATCAACG30450.041.9781273
CAACGCA31350.041.0728155
AACGCAG33000.039.0191736
GGTATCA9350.036.2477871
TATCAAC40600.032.2957762
TTAGGTA5800.031.599344
GTACATG62150.031.0529861
AGGTATA5500.030.7596426
CTTAGGT5850.030.5259463
ACGCAGA42100.030.2502277
TACATGG63600.030.001052
TAGGTAT5900.029.4707495
ACATGGG65300.029.0743243
GTCTTAG7700.028.7321241
CGCAGAG46150.027.9195148
GCAGAGT50700.025.692179
GGTATAG6850.024.697527
ACCTAAG7350.024.336431