FastQCFastQC Report
Sun 14 Apr 2019
SRR6575912_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575912_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1698265
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA23710.13961307569784456No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC22280.13119271727321707No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG22250.13101606639717595No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT21970.12936732488745867No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC21880.12883737225933525No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA20950.12336119510206005No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA20440.12035813020936073No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC20330.11971041033054323No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA19080.11234995716216255No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA17700.1042240168642703No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT17560.10339964610941166No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA26300.052.1938741
GGTATCA9550.043.810581
TCAACGC32550.040.8613554
ATCAACG33600.039.724313
CAACGCA34150.038.9469155
AACGCAG35700.037.5192346
ACCTAAG9550.033.9655081
TATCAAC43000.031.37662
GTACATG68850.030.4525261
TACATGG69350.029.9618232
TAGGTAT7850.029.9348895
AGGTATA7800.029.5242446
ACATGGG71600.029.2095133
TTAGGTA8400.029.0938634
TAAGACA14550.029.0707914
CTAAGAC13750.029.0531713
ACGCAGA45950.029.0475947
GTATAGA2600.028.9293731
GTCTTAG10800.028.7284761
CTTAGGT7900.027.9607033