FastQCFastQC Report
Sun 14 Apr 2019
SRR6575912_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575912_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1698265
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA25910.15256747327419456No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC21150.12453886760900096No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC20920.1231845442260189No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG19490.11476418580139143No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC19450.11452865130000324No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT18860.11105451740452757No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA18080.10646159462745802No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT17710.10428290048961734No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA27200.048.5240141
TCAACGC34100.037.4921074
ATCAACG34200.037.3835833
CAACGCA35700.036.0740475
GGTATCA11200.035.7740821
AACGCAG36550.035.1065256
CTTAGGT6800.032.4883043
TTAGGTA6650.031.8065264
TAGGTAT7350.030.6949165
GTACATG68600.029.7530421
TATCAAC44050.029.3443782
GTCTTAG10350.029.1478961
TACATGG69350.029.0769582
TTAACGC650.006152197328.9257583
AGGTATA7150.028.9240576
ACATGGG70150.028.3433253
ACCTAAG10700.027.7539221
ACGCAGA46800.027.314757
GGTATAG7750.026.6824177
CGCAGAG49300.025.9303848
Produced by FastQC (version 0.11.2)