FastQCFastQC Report
Sun 14 Apr 2019
SRR6575913_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575913_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1488517
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA22420.1506197107590978No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG20970.14087847166004822No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC20220.13583989971226396No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC20120.13516809011922604No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT18920.1271063750027712No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA18360.12334424128175896No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA17520.11770104070024058No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA17470.1173651359037216No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC17430.11709641206650645No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA15710.10554128706625453No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA26200.050.235131
TCAACGC32200.039.836624
ATCAACG33050.038.8120773
CAACGCA33250.038.578625
AACGCAG34000.037.8658186
CTTAGGT6150.037.4366233
GTCTTAG8450.037.2708171
TAGGTAT6450.036.423865
GGTATCA10650.035.7508351
AGGTATA6400.035.2400866
TTAGGTA6450.034.9669044
TTACGGT600.004167104631.3245224
GGTATAG7650.030.7103167
TATCAAC43350.030.2528252
TCTTAGG9950.029.292
ACGCAGA44100.029.0870557
GTACATG64000.028.8644561
TACATGG64450.028.0794532
CGCAGAG46800.027.5093568
ACATGGG65650.027.1972243