FastQCFastQC Report
Sun 14 Apr 2019
SRR6575913_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575913_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1488517
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA24100.16190611192213458No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC20400.13704915697973216No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC20240.13597426163087153No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG19130.1285171751481508No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT17770.11938056468283532No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC17530.11776822165954436No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA16970.1140060879385321No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT15440.1037274011650522No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA15250.10245096293828018No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA15250.10245096293828018No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA23800.051.2272531
TCAACGC30000.039.787454
GGTATCA9750.039.5901871
ATCAACG30400.039.1093443
CAACGCA30500.038.9811175
AACGCAG31650.037.4162676
TATCAAC39100.031.010282
GTCTTAG9950.030.2785571
TTAGGTA7650.029.4858344
GTACATG58600.029.4813061
AGGTATA7200.029.3706556
TAGGTAT7200.029.3706555
CTAAGAC10400.028.9187983
ACGCAGA41200.028.7433197
ACCTAAG8100.028.4766791
CTTAGGT7600.028.443163
TAAGACA11900.028.4327684
TACATGG60200.028.2601762
ACATGGG61150.027.4350243
CGCAGAG44150.027.0538148