FastQCFastQC Report
Sun 14 Apr 2019
SRR6575914_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575914_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences534365
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG11490.21502156765506722No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA6730.12594387731232398No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5870.10985000888905523No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA5860.10966287088413351No Hit
CCTATAAGCAGTTCTTGTATTTTTATTCACACAGGTCTGATTAAGATGAA5650.10573297278077719No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT5430.10161593667249913No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTTAG250.005239856456.385461
GTATCAA11850.054.7200621
ATCAACG15700.041.0021633
TCAACGC15850.040.6141324
AACGCAG15950.040.3594976
CAACGCA16350.039.372115
GTACATG31150.037.8618241
TACATGG31150.037.710982
ACATGGG31700.037.353143
TATCAAC18700.034.424282
ACGCAGA18650.034.264627
CGCAGAG19450.032.8552788
CATGGGG15500.031.5273514
AAGACCG600.00416425931.3252565
ATGGGGA8550.031.3252545
GGTATCA4750.030.6657751
GTAATAC953.7551614E-529.6765583
CTCGTCG650.00615901428.915629
GCAGAGT22100.028.7030039
CATGGGA13950.028.630614
Produced by FastQC (version 0.11.2)