FastQCFastQC Report
Sun 14 Apr 2019
SRR6575914_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575914_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences534365
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG11430.21389873962553685No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7790.1457805058340273No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA6970.13043518943044546No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA6410.11995546115482862No Hit
CCTATAAGCAGTTCTTGTATTTTTATTCACACAGGTCTGATTAAGATGAA6100.11415418300225501No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT6060.1134056309825681No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTG5440.10180307467742088No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA15200.055.736031
ATCAACG19950.042.162453
CAACGCA20200.041.8732645
TCAACGC20200.041.6406364
AACGCAG20600.041.2883076
ACGCAGA23800.035.7369357
TATCAAC23800.035.5394942
GTACATG33050.035.0324671
CGCAGAG24950.034.300558
TACATGG34050.034.2255362
ACATGGG34250.034.162883
GCAGAGT27800.031.1224169
CATGGGG14200.029.7830984
ATGGGTA5750.029.4205235
GGTATCA6500.028.2395881
CATGGGT9550.027.0629434
TATAGAG1807.494236E-926.1061745
GGTACCT5800.025.9431238
ATGGGGA8000.025.8451125
CATGGGA16000.025.5514184
Produced by FastQC (version 0.11.2)