FastQCFastQC Report
Sun 14 Apr 2019
SRR6575917_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575917_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1683117
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA34560.20533331907407504No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC29980.17812190121067045No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC28880.17158640783736365No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT21470.12756094793172432No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA20860.12393671978834508No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG20570.12221372608083692No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC20420.12132252243902238No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA19990.11876773866582062No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA19890.11817360290461092No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA19290.11460878833735265No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA28050.052.1167951
TCAACGC36100.039.4476434
ATCAACG36300.039.23033
CAACGCA37000.038.615135
AACGCAG38450.037.4033666
TAGGTAT9300.034.3646245
GGTATCA12000.034.07911
GTCTTAG12050.031.9872531
TTAGGTA10000.031.95914
ACCTAAG14750.031.8682421
TCTAACG600.004159073331.3371092
AGGTATA10200.031.3324536
TATCAAC46300.031.16792
CTTAGGT10400.030.7299063
CTAAGAC16700.030.1129263
ACGCAGA49050.029.3202787
TAAGACA17900.029.1444324
GTACATG71400.029.0987421
ACCAGAT16200.029.01152894
CCTAAGA17200.028.6953162
Produced by FastQC (version 0.11.2)