FastQCFastQC Report
Sun 14 Apr 2019
SRR6575917_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575917_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1683117
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA36570.2172754478743902No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC29460.17503239525237996No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC28690.1704575498910652No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC20350.12090662740617557No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA20310.12066897310169168No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG20120.11954011515539324No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT19930.11841125720909479No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT19420.11538116482692527No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA17900.10635030125653773No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA17290.10272607311315851No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCG16840.10005246218771481No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA27950.050.0754281
GGTATCA11550.041.6167561
TCAACGC33800.039.9132424
CAACGCA34550.038.9107675
ATCAACG35050.038.4898033
TATAGCG757.439312E-637.604675
AACGCAG36150.037.1885766
GTCTTAG12000.034.9509351
AGGTATA9750.033.7477766
TTAGGTA10000.033.374154
TAGGTAT10050.033.2081075
CTTAGGT10150.032.880933
TATCAAC42400.032.3719442
GTACATG66200.030.3250581
ACGCAGA44750.029.9313437
GGTATAG11050.029.7721487
TACATGG68000.029.5169032
TCTTAGG13700.028.8210962
ACATGGG69650.028.345233
TATAGTA11700.028.1189739