FastQCFastQC Report
Sun 14 Apr 2019
SRR6575918_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575918_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1512739
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA34170.22588166233567059No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC30030.19851408603863588No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC27090.17907914055233587No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT19910.1316155662014399No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA19540.12916967170146337No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG18870.12474061949880318No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA18220.1204437778096552No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA18150.11998104101236234No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA17300.11436209418809193No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC16980.11224672597189601No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT16560.10947030518813888No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT15390.10173599014767253No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA26650.046.5663221
GGTATCA11050.038.7118871
TCAACGC31550.038.4236764
ATCAACG31450.038.2470473
CAACGCA32950.036.933715
AACGCAG34550.035.223326
TTAGGTA8850.035.041224
CTTAGGT8900.034.3164183
AGGTATA9000.033.4130446
TAGGTAT9150.033.3788075
GTCTTAG10550.032.971941
GGTATAG9350.032.162297
GTACATG62850.029.917131
TATCAAC41000.029.6948492
GTATAGG2400.029.3795591
TCTTAGG12200.029.2832322
ACCTAAG10350.029.067311
TATAGTA10750.028.847899
TACATGG65300.028.7946642
GTATAGT10900.028.45098