FastQCFastQC Report
Sun 14 Apr 2019
SRR6575918_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575918_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1512739
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA36110.23870608214635836No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC28020.18522692943065525No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC27400.18112840351177567No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG19470.1287069349041705No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT19190.1268559877149991No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC17510.1157503045799705No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA17300.11436209418809193No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA17150.1133705153367501No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA16970.11218062071513989No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT16730.11059409455299295No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA23550.052.5948941
TCAACGC29650.040.732274
ATCAACG29800.040.6849373
CAACGCA30550.039.6861235
AACGCAG32400.037.5651366
GGTATCA9400.037.0751231
GTCTTAG9900.033.2997931
GTACATG62150.031.7505781
CTTAGGT9050.031.1553653
TACATGG63150.030.8829862
ACGCAGA39250.030.7697267
TTAGGTA9100.030.4677814
TATCAAC40000.030.1937962
ACATGGG64100.030.1310313
TAGGTAT9150.029.2741285
AGGTATA9400.028.495566
CGCAGAG43950.027.4983088
GGTATAG10050.026.6525637
ACCTAAG12450.026.1010781
TCTTAGG12450.025.667532