FastQCFastQC Report
Sun 14 Apr 2019
SRR6575920_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575920_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1184400
Sequences flagged as poor quality0
Sequence length100
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA25590.21605876393110435No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT24580.20753123944613305No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG19300.162951705504897No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA18770.15847686592367444No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT18290.15442418101992572No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA18010.1520601148260723No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA16580.13998649105032085No Hit
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC15510.13095238095238096No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA15500.1308679500168862No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT14590.12318473488686255No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC14050.11862546437014522No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA14030.1184566024991557No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT13850.11693684566024991No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCG13650.11524822695035461No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA13450.11355960824045931No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT13200.11144883485309016No Hit
GAATAATGGAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAG12780.10790273556231005No Hit
GTTATTGCTCAATCTCGGGTGGCTGAACGCCACTTGTCCCTCTAAGAAGT12360.10435663627152988No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA12140.10249915569064505No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGATCTTGGGAGCGG12060.10182370820668693No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT11870.1002195204322864No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAG4650.035.3816681
ACCTAAG3500.032.233421
CCTAAGA4900.028.7822742
TAGGCAT6750.028.5415975
TAAGACA6400.026.431434
CTAAGAC5700.026.3799083
CATGGGG32800.026.3598024
TCTAACG2500.026.3261872
GGTAATC1458.668176E-725.9250858
CTAACGC2550.025.7983
GGGTACC8050.025.0998277
ATGGGGG14900.024.9137425
CAATGCG3050.024.64799918-19
TGGGTAC8400.024.6133986
TAGTACT6900.024.516114
GTATATG2501.6370905E-1124.4436781
GGTACCT8300.024.343818
ACATGGG76150.023.6334463
GTACATG77300.023.5339411
TAGGTAT2402.6375346E-1023.4946065
Produced by FastQC (version 0.11.2)