FastQCFastQC Report
Sun 14 Apr 2019
SRR6575925_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575925_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences285667
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG6800.23803939551995854No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC4210.1473743904616214No Hit
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA4170.14597415872326872No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC3990.13967311590068154No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA3850.1347723048164471No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA3620.1267209723209191No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3190.11166848113362761No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT3040.106417612114805No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA2980.10431726450727595No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA2940.10291703276892325No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAACTC652.774912E-643.3905942
CCGAACT901.2982127E-841.8128171
CCCGCTA450.001012860641.7762223
CCGCTAA500.001700197937.5986024
ATTTAGG550.002702472534.2104871
CGCTAAA550.002714102634.1805465
GCCTAAA600.004138774231.3596121
GTACATG17100.030.8094441
TACATGG17500.030.0841462
ACATGGG17250.029.4249923
TCTAGGG650.00614765228.9220033
GTATCAC650.00614765228.9220033
TCTAGAT1802.3646862E-1028.7261832
CATGGGG9000.028.198954
GTATCAA8850.027.638981
GCAAACG700.00878909226.879671
TAGCACA700.00882671326.8561444
ATCGGAT350.00883397326.85614492-93
CTAGGCA700.00882671326.8561444
ATACCGT1057.404276E-526.8561426