FastQCFastQC Report
Sun 14 Apr 2019
SRR6575927_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575927_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1124132
Sequences flagged as poor quality0
Sequence length100
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA19650.17480153576270402No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT18010.16021250173467172No Hit
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC17050.15167257937679918No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT14120.12560802468037563No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA13770.12249451132073456No Hit
GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC12710.11306501371725029No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA12320.10959567025936455No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT12000.10674902947340704No Hit
GTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGG11650.103635516113766No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT11540.10265698334359309No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCG11260.10016617265588028No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCTAAG3850.031.746331
TTAGGTA2750.030.7626784
GTACTAG4450.030.635071
AGGTATA2350.029.9990636
TAGTACT8400.027.9753174
CTAGTAC8500.027.6461943
CCTAAGA5000.027.2652132
CATGGGG27800.026.2042184
CTAAGAC6550.025.8312553
CCTAGTA9050.025.452382
TAAGACA7050.024.6658954
TACTCTC1151.377474E-424.5209735
GTATCAA38050.024.2148781
GTACTGT9000.024.0214716
TAGGTAT2950.023.8975585
CTGTGCG9200.023.4992669
TACATGG60750.022.982172
ATGGGGG16350.022.708775
CGTTAAC1452.5294687E-522.6939951
ACATGGG61200.022.6545223
Produced by FastQC (version 0.11.2)