FastQCFastQC Report
Sun 14 Apr 2019
SRR6575928_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575928_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1044274
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT24660.23614491981989402No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA23090.22111055144531036No Hit
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC16670.15963243363331844No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA13660.13080858088968988No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT13020.12467992117011437No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT12470.11941310422360414No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT12230.11711485682876334No Hit
GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC11710.11213532080660822No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCG11540.11050739556859598No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA11500.11012435433612251No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG11300.10820914817375517No Hit
CAATTGATATTAGATATTTTTGCAAAGCGGGCGAGAACGAGAGAAGGCAA10850.10389993430842863No Hit
GACCATAATGTGATCCCTTCCGGCGGTCGGTATAAAATCAGGCAGTTTTT10780.10322961215160006No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA10650.10198472814606128No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTAAC1501.0495569E-931.389051
GTACTAG4350.030.3066671
TCTAACG2600.027.1128732
GTATAAG4700.027.0480121
TAGTACT7050.026.6629184
GTCTATA2156.366463E-1126.2792051
ACCTAAG4550.024.835291
CTAACGC2850.024.7333643
GTACTGT7700.024.4109846
CATGGGG26150.024.2604524
TATAAAA9100.023.7560392
CTGTGCG8400.023.511339
TAGGCAT7800.022.8942155
GTATCAA38100.022.8621031
TGATCGC5200.022.607059
CTAGTAC8000.022.3218613
CTTAGTG1503.2945736E-521.930253
CGATAAT4100.021.78733666-67
GCTATCC1303.1490994E-421.7027688
ACGCCTA3251.8189894E-1221.688226
Produced by FastQC (version 0.11.2)