FastQCFastQC Report
Sun 14 Apr 2019
SRR6575931_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575931_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences750708
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA16160.2152634579623502No Hit
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA13680.18222797678991032No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT13310.1772992961311189No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA11210.1493257032028432No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC10140.13507249156795986No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9260.12335022405515861No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT8690.11575739168891233No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA8670.11549097651816684No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA8260.11002946551788445No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC8000.10656606829819318No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA7830.10430153934685657No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA33600.033.295841
TAGGTAT3000.032.8997465
AGGTATA3100.030.3223446
TTATCCT7500.030.0797654
TTAGGCC1102.966819E-629.9088574
CTTAGGT3150.029.8410383
GTACTTG4600.029.6341511
AAGGGTA2250.029.2442175
TCTAACG1154.1897492E-628.6122842
ATGGGAG9600.028.395615
CATATAG1503.6945494E-828.1997813
CTATTAT6850.028.1349161
CATGGGG22250.027.460464
TATTATC7200.027.4201052
TTAGGTA3650.027.0408864
GTACATG63900.026.8500041
ACATGGG62150.026.7705083
CAACGCA42100.026.5698645
GTCTTAG4250.026.5445061
TACATGG64050.026.4201762
Produced by FastQC (version 0.11.2)