FastQCFastQC Report
Sun 14 Apr 2019
SRR6575932_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575932_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences693596
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA17440.2514432032480003No Hit
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA14230.2051626595309085No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT12730.18353623723320203No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA9530.13739986966476161No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC9360.13494887513768822No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9000.12975853378623867No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG8140.11735938500222032No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC7470.1076995830425781No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA7220.1040951793262937No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTTTAT2350.032.0293581
TAGGTAT2350.031.994725
ACCTAAG4900.031.68211
ACCGTCC650.00613580828.9391698
CTAAGAC6350.028.8613823
TCTAACG1154.198624E-628.6039772
GTATCAA36950.028.51871
GGTAATC1652.9431249E-928.50078
AGGTATA2500.028.1953496
TAAGACA6950.027.0458984
CCTAAGA6650.026.8527132
GTACATG66100.026.759781
GGTATAG3000.026.628947
CTTAGGT2650.026.5993883
AAGGGTA1956.0572347E-1026.508455
ACATGGG66050.026.3241983
TACATGG68200.025.9766562
TGATCGC1458.6003274E-725.9454619
CATGGGG23400.025.7051624
CTAATGT1101.0189069E-425.632144
Produced by FastQC (version 0.11.2)