FastQCFastQC Report
Sun 14 Apr 2019
SRR6575933_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575933_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1126646
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA42780.3797111071268171No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC33530.29760900939603036No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC30510.27080378397473565No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG15090.13393736808189974No Hit
GTTTGGGACTGACCCTAGTCTGTGTCCATGCAGAAGAAGCTAGTTCTACG14670.13020948904979915No Hit
ATCCTAGGTAACATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAG14180.12586029684568178No Hit
ACCCTAGTCTGTGTCCATGCAGAAGAAGCTAGTTCTACGGGAAGGAACTT13440.11929212902721885No Hit
ATCCTATTCCCTACCAAAATGAAGATGCTGCTGCTGCTGCTGTGTTTGGG13370.11867081585520207No Hit
GAATTTATACTGGGGATGCTGTATGGATAGGAAGGGATGATGGTGGAGTC12390.10997243144696736No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG11410.10127404703873266No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA32750.044.8779871
ATCAACG43150.033.8739783
AGGTATA8800.033.6468546
TCAACGC43450.033.6400954
CAACGCA44300.033.100725
GTCTTAG9950.032.6675151
AAGGGTA3050.032.3598145
TTAGGTA9200.032.1839454
TAGGTAT9400.031.9991685
GTACATG78250.031.9668791
AACGCAG45950.031.9121196
CTTAGGT9150.031.8461673
GGTATAG9300.031.3311317
TATCAAC46950.031.2324162
TACATGG80400.030.864872
ACATGGG79350.030.7402233
TCTAACG650.006154896728.9223272
CATGGGG34650.028.6197474
TCTTAGG11650.028.239612
ACCTAAG15550.027.5677341