FastQCFastQC Report
Sun 14 Apr 2019
SRR6575934_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575934_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1021493
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA35090.34351679355609877No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC31940.31267957783362194No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC29890.29261091363327996No Hit
ATCCTAGGTAACATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAG15790.154577662304098No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG15220.14899759469717364No Hit
ACCCTAGTCTGTGTCCATGCAGAAGAAGCTAGTTCTACGGGAAGGAACTT13620.13333424702861402No Hit
GAATTTATACTGGGGATGCTGTATGGATAGGAAGGGATGATGGTGGAGTC13480.13196370410761504No Hit
GTTTGGGACTGACCCTAGTCTGTGTCCATGCAGAAGAAGCTAGTTCTACG13140.1286352427280461No Hit
ATCCTATTCCCTACCAAAATGAAGATGCTGCTGCTGCTGCTGTGTTTGGG11040.10807709891306157No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT11010.10778341114427607No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA10850.1062170763774201No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA10720.10494442937934963No Hit
GTGTAGCCACGATCACAAGAAAGACGTGGTCCTGACAGACAGACAATCCT10480.1025949272290657No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA35600.041.5887031
AGGTATA8550.034.0715266
TCAACGC43350.033.8183754
ATCAACG43650.033.585953
CAACGCA43650.033.369025
AACGCAG44950.032.4039546
TAGGTAT9050.032.189125
CTTAGGT8950.032.0253643
TATCAAC48100.030.4876752
GTCTTAG10100.030.2487091
TTAGGTA9500.030.1712634
GTACATG70600.029.1597791
GGTATAG10050.028.9862217
TCTTAGG11550.028.079022
ACATGGG71900.028.0359753
ACGCAGA52350.027.823457
TACATGG73200.027.7387872
CGCAGAG53350.027.3019248
ACCTAAG12600.027.2312221
GGTATCA18900.027.1068781