FastQCFastQC Report
Sun 14 Apr 2019
SRR6575934_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575934_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1021493
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA42420.4152745050626876No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC30480.29838677308606126No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC28000.27410858419979384No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG13890.13597743694768344No Hit
GTTTGGGACTGACCCTAGTCTGTGTCCATGCAGAAGAAGCTAGTTCTACG13240.1296142019573311No Hit
ATCCTAGGTAACATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAG12950.12677522019240464No Hit
ATCCTATTCCCTACCAAAATGAAGATGCTGCTGCTGCTGCTGTGTTTGGG12240.1198246096644813No Hit
ACCCTAGTCTGTGTCCATGCAGAAGAAGCTAGTTCTACGGGAAGGAACTT11730.11483191759512792No Hit
GAATTTATACTGGGGATGCTGTATGGATAGGAAGGGATGATGGTGGAGTC11070.10837078668184706No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG10430.10210544761442321No Hit
GTGTAGCCACGATCACAAGAAAGACGTGGTCCTGACAGACAGACAATCCT10310.10093069653928123No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA30450.044.9526861
GTACTAG801.15210405E-535.278591
TCAACGC39100.034.2558864
ATCAACG39450.034.07113
CAACGCA39350.033.918825
TAGGTAT7350.033.2493485
AACGCAG40650.032.946476
AGGTATA7800.031.9305116
GTCTTAG8500.031.5432111
CTTAGGT7750.031.533253
TGTCGCG600.00414826631.35260294
TATCAAC44000.030.8682692
GTACATG67200.029.9588031
GGTATAG8200.029.799857
TACATGG70600.028.3577652
ACATGGG69400.028.1709143
TTAGGTA8900.027.4587294
ACGCAGA48850.027.319857
ACCTAAG13500.027.1775821
TCTTAGG10050.027.122462