FastQCFastQC Report
Sun 14 Apr 2019
SRR6575936_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575936_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences981260
Sequences flagged as poor quality0
Sequence length100
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA11080.11291604671544747No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT10430.1062919104009131No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT10280.10476326355909747No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG10130.10323461671728185No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA16050.058.072561
GGTATCA6000.055.7041931
TCAACGC22350.040.9984
ATCAACG22900.040.013333
CAACGCA23350.038.839715
AACGCAG24400.037.3628126
TATCAAC27700.033.5919532
ACGCAGA29700.030.5371537
GTCTTAG4200.030.2618351
CTTAGGT2750.029.0483723
CGCAGAG32500.027.9247598
GTAACGG1057.3900876E-526.87334494
GTACATG39300.026.8309651
TACATGG40000.026.1996022
GCAGAGT34900.025.8696949
ACATGGG40950.025.474443
AGAGTAC29800.025.0897510-11
TCTTAGG4500.024.0195662
AGGTATA2352.0190782E-1023.9961246
TAGGTAT2352.0190782E-1023.99495
Produced by FastQC (version 0.11.2)