FastQCFastQC Report
Sun 14 Apr 2019
SRR6575937_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575937_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences299633
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5190.17321189588596717No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG5040.16820577172741322No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA8100.051.1309131
ATCGAGC205.660039E-447.02438492-93
ATCAACG11050.035.2979053
TCAACGC11100.035.1389054
AACGCAG11300.034.9328426
CAACGCA11450.034.064795
CATGGGT5000.031.9552354
TATCAAC12500.031.5792882
GGTATCA4350.031.3757861
GTACATG17150.031.009891
TACATGG17000.030.9600872
ACATGGG17450.030.161693
CGCAGAG13400.029.82898
ACGCAGA13450.029.3487827
TAGCACC856.810184E-427.6429354
ACATCGT700.00880348626.8710758
GCAGAGT15950.025.0600179
GTACTTT10850.024.7246991
ATGGGTG2552.1827873E-1123.9572125
CATGGGG5900.023.8947414
Produced by FastQC (version 0.11.2)