FastQCFastQC Report
Sun 14 Apr 2019
SRR6575938_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575938_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1082016
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA22730.2100708307455712No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC17850.16496983408748114No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC12890.11912947682843876No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA11380.10517404548546418No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA11040.10203176293141691No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA5550.061.8217961
GTATCAA18300.056.504541
TCAACGC24550.041.160314
CAACGCA25900.039.922215
ATCAACG25500.039.6268843
AACGCAG27300.038.0470736
TATCAAC29800.034.3836482
GTACATG47900.032.2828671
TACATGG49300.031.6521282
ACATGGG49900.030.9875353
ACGCAGA34350.030.3751137
ACCTAAG11300.029.1159761
TAGGTAT4900.028.7750995
TAAGACA12750.028.752534
CGCAGAG36650.028.4688998
CTAAGAC12650.027.8652173
CCTAAGA12250.027.6253742
TTAGGTA5800.027.551334
AGGTATA5100.026.725116
CTTAGGT5850.026.5124423
Produced by FastQC (version 0.11.2)