FastQCFastQC Report
Sun 14 Apr 2019
SRR6575938_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575938_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1082016
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA21830.2017530239848579No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC17670.1633062727353385No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC13510.12485952148581907No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA19850.060.308751
GGTATCA6350.049.7288321
ATCAACG27450.043.6635633
TCAACGC27550.043.1638574
CAACGCA28300.041.8538555
AACGCAG29250.040.4944956
TATCAAC31800.037.3951032
ACGCAGA36450.032.492587
AGGTATA5150.031.0308066
CGCAGAG38950.030.407058
TAGGTAT5250.029.5444555
ACCTAAG10400.029.456911
GTCTTAG6650.029.0582431
CTTAGGT5400.028.7237763
CTAAGAC10900.028.4602533
GTACATG45550.027.9371781
GCAGAGT42650.027.658969
TACATGG46000.027.2819122
GGTATAG5750.026.972887
TTAGGTA5800.026.7428254
Produced by FastQC (version 0.11.2)