FastQCFastQC Report
Sun 14 Apr 2019
SRR6575939_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575939_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences970322
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA20400.2102394875103316No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC16660.17169558146677083No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC12960.13356390971244597No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA9910.10213104515820523No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA18250.060.5097961
GGTATCA6050.054.3705021
TCAACGC24600.044.1217584
ATCAACG24900.043.401473
CAACGCA25950.041.8264055
AACGCAG26850.040.42446
TATCAAC27850.038.9769172
TACGGTA550.002719167934.1757472
ACGCAGA32150.033.61427
CGCAGAG34000.031.7851948
TAACCCG1401.8955689E-830.2058075
GCAGAGT38300.028.2166219
GTACATG40400.027.68321
TACATGG41650.026.5138972
CTTAGGT4600.025.5363063
ACATGGG43250.025.3131223
TATGGGT1501.1650645E-625.0596314
AGAGTAC33250.024.44727110-11
TAGGTAT5050.024.1912295
TTAGGTA5100.023.9540584
Produced by FastQC (version 0.11.2)