FastQCFastQC Report
Sun 14 Apr 2019
SRR6575939_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575939_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences970322
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA22720.2341490762860164No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC16490.16994358573751805No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC13890.14314835693718167No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA10570.10893291093059829No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA19150.065.908121
GGTATCA4900.049.9781461
ATCAACG26050.047.6242453
TCAACGC26250.047.2613954
CAACGCA27400.045.2777945
AACGCAG27950.044.554956
TATCAAC31300.039.9364282
ACGCAGA33900.036.596217
CGCAGAG35650.034.8212978
GCAGAGT40000.031.0344819
ACCTAAG8350.030.4565121
ACTACGC1303.3351353E-728.9186843
GTCTTAG6250.028.6336331
GTACATG42400.028.1013771
GTATAGA1354.5525303E-727.9080241
CTAAGAC9600.027.9020143
TACATGG43950.026.9447122
TAAGACA10750.026.6657184
ACATGGG44350.026.2778593
AGAGTAC35450.026.26331110-11
Produced by FastQC (version 0.11.2)