FastQCFastQC Report
Sun 14 Apr 2019
SRR6575940_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575940_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences801617
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA29350.3661349497328525No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC16620.20733093235298156No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11960.14919843266796987No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC10970.13684839518123992No Hit
GAATTTATACTGGGGATGCTGTATGGATAGGAAGGGATGATGGTGGAGTC9480.11826096502444434No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT9280.1157660079564181No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA9230.11514226868941152No Hit
ATCCTAGGTAACATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAG9130.1138947901553984No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA9020.11252256376798396No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC8420.10503769256390522No Hit
GTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCA8100.10104576125506319No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA25050.059.623491
GGTATCA8650.057.1924931
ATCAACG34250.043.092743
TCAACGC34700.042.53394
CAACGCA34850.042.483055
TATCAAC35300.042.210412
AACGCAG36300.041.0450336
ACGCAGA42500.034.721187
CGCAGAG42650.034.709258
GCAGAGT45150.032.5791979
GGTAATC2600.030.7276488
ATGGGAG9600.029.8652765
GTACATG49600.029.6373061
AAGGGTA2550.029.490885
CTTAGGT4700.029.0024833
TACATGG51100.028.8830992
ACATGGG51100.028.6991313
AGGTATA4750.028.6954026
TAGGTAT4850.028.1037455
TCTTAGG6550.027.987132