FastQCFastQC Report
Sun 14 Apr 2019
SRR6575941_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575941_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences744135
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA27730.3726474362850827No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC15160.20372647436285082No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC11120.14943525032420193No Hit
ATCCTAGGTAACATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAG10220.1373406707116316No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA10190.13693751805787927No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10110.1358624443145397No Hit
GAATTTATACTGGGGATGCTGTATGGATAGGAAGGGATGATGGTGGAGTC10040.13492175478911755No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA9240.12417101735572175No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC8270.11113574821772931No Hit
GTGCAAGGGTAATCATTTATTGAACAGGAAGAGGAAGAAATTCATGAAAA8100.1088512165131327No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA7740.10401338466810457No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT7650.10280392670684756No Hit
GTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCA7450.10011624234849859No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA22600.060.128731
GGTATCA7500.051.4097181
ATCAACG31950.041.618563
TCAACGC32400.041.3305664
CAACGCA32500.041.0588235
AACGCAG34650.038.917976
TATCAAC34750.038.834682
CGCAGAG39050.034.051558
ACGCAGA39350.033.7919467
GGCGTCG1554.1836756E-1133.345148
ACCTAAG10300.032.8691061
GCAGAGT41450.031.8532169
TAAGACA12450.030.9468174
GTACATG45500.030.7962251
TACATGG45950.030.2899672
ACATGGG45650.030.0547813
CTAAGAC11950.029.8825153
CATGGGA21850.028.8154134
GTGTGCG1005.3316064E-528.1917959
CCTAAGA12550.028.1002482
Produced by FastQC (version 0.11.2)