FastQCFastQC Report
Sun 14 Apr 2019
SRR6575941_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575941_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences744135
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA27920.3752007364255142No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC14160.19028805257110604No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC11860.15937968245009307No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9890.13290599152035584No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA9350.12564924375281367No Hit
GAATTTATACTGGGGATGCTGTATGGATAGGAAGGGATGATGGTGGAGTC9080.12202086986904258No Hit
ATCCTAGGTAACATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAG9070.12188648565112513No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT8540.1147641221015004No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA8440.11342027992232591No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC7680.1032070793605999No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA23200.061.8954851
GGTATCA7300.047.0811581
ATCAACG32350.043.5818253
CAACGCA32750.043.0495265
TCAACGC33900.041.7277764
AACGCAG34500.041.4107326
TATCAAC35300.040.608112
GTACTAG1750.040.3552781
ACGCAGA39850.035.3770987
CGCAGAG40150.035.1340078
GGTAATC2950.035.0665178
GCAGAGT43000.032.9147079
AAGGGTA3050.032.3577165
ACCTAAG9550.029.5797841
GTGCAAG3400.029.0795361
TGTCGAC650.00615754328.9185097
GTACATG45650.028.5684151
TAGGCAT3150.028.346635
AGGTATA4550.027.8875796
TACATGG46250.027.8436662