FastQCFastQC Report
Sun 14 Apr 2019
SRR6575943_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575943_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences998207
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA13230.13253763998849938No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA13170.13193656225612524No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT12200.12221913891607653No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG11610.11630854121439742No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT11390.11410458952902554No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA11350.11370387104077612No Hit
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA10570.10588986051991221No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC10360.10378608845660268No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA10300.10318501072422853No Hit
GTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCA10160.10178249601535552No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT9990.10007944244029546No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA16800.061.3039671
GGTATCA6050.052.857581
TCAACGC24150.041.4425244
ATCAACG24550.040.5758973
CAACGCA24900.039.8168455
AACGCAG25550.038.9877976
GTACTAT1101.7862476E-938.477211
TATCAAC29500.033.7944832
ACGCAGA31150.031.9787567
GTACATG42600.030.9102271
CGCAGAG32850.030.4668798
TATAGGG1701.2187229E-1030.4281652
TACATGG42850.030.1796252
ACATGGG44200.029.2343983
GCAGAGT36400.028.0104669
GTACTAG1057.390913E-526.872971
CATGGGG25600.026.4305554
AGAGTAC32500.025.5166310-11
CCTTTAT3400.023.5138511
GTATAGG2905.456968E-1222.7030281
Produced by FastQC (version 0.11.2)