FastQCFastQC Report
Sun 14 Apr 2019
SRR6575943_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575943_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences998207
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT11860.11881303176595635No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA11670.11690961894677157No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA11660.11680943932470919No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG11610.11630854121439742No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT11300.11320297293046433No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA10740.10759291409497228No Hit
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA10610.10629057900816163No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA16950.058.0629161
GGTATCA5500.054.7948231
TCAACGC23050.041.7959334
ATCAACG23750.040.3641553
CAACGCA23900.040.3094675
AACGCAG24700.038.8136336
TATCAAC29850.032.7485352
ACGCAGA30400.031.53457
GTACATG40350.029.9924411
TAGGTAT3200.029.3718385
ACCTAAG4700.029.0551011
CGCAGAG32950.028.9702938
TACATGG40900.028.7269542
ACATGGG42150.027.9837253
GCAGAGT35600.027.077979
AACCACG1057.4285825E-526.8529077
AGGTATA3550.026.4760236
GTCTTAG5050.026.1089211
GTGTTAT2357.2759576E-1226.04941
CATGGGG25400.025.9027214
Produced by FastQC (version 0.11.2)