FastQCFastQC Report
Sun 14 Apr 2019
SRR6575944_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575944_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences631531
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA12430.1968232755003317No Hit
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC11740.18589744604777914No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT11390.18035535864431043No Hit
GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC10500.1662626221040614No Hit
CAATTGATATTAGATATTTTTGCAAAGCGGGCGAGAACGAGAGAAGGCAA9360.14821125170419186No Hit
GACCATAATGTGATCCCTTCCGGCGGTCGGTATAAAATCAGGCAGTTTTT9100.14409427249018655No Hit
CCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAACTTTTCAGGCGAA8590.13601865941656072No Hit
GTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGG8350.13221837091132502No Hit
GTATAAGGTGATCGCAGGTTGTGCAATCATTGCTCAAAAGGGTGTACACC7720.12224261358508134No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA7360.1165421808272278No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC7020.1111584387781439No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC6880.10894160381675641No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA6730.10656642350098412No Hit
CGTTTATTGTAAGCCGTCAGCATCGGGATATCATCTGCTTCAAGCTCCTC6610.10466627924836627No Hit
GTTAATAATTCTGGCAATTCGTCTCCACACTAGAAGTCGACGAACAACGA6490.10276613499574842No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6370.10086599074313057No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA17850.050.3100851
CAACGCA22100.040.4063225
ATCAACG22450.039.5670283
AACGCAG25500.036.8619086
GGTATCA8250.036.4742161
ACGCAGA25900.034.6594437
CGCAGAG26300.034.1323058
AGGTATA702.1841831E-433.5706676
TAGGTAT702.1841831E-433.5706675
GCAGAGT27550.033.0954369
CTACCCC1001.4100806E-632.899254
TATCAAC27700.032.7595182
TCAACGC27300.032.709884
TACCCCG902.5924905E-531.332625
GTACATG29400.029.745791
TAACCAC2250.029.2437785
TACATGG29650.029.1778342
CTATTGA5200.028.922429
GGGTACC3750.028.826017
ACATGGG30100.028.4179573
Produced by FastQC (version 0.11.2)