FastQCFastQC Report
Sun 14 Apr 2019
SRR6575944_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575944_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences631531
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA12440.19698162085471657No Hit
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC12290.19460644053894424No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT11880.1881142810091666No Hit
GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC10040.15897873580235966No Hit
CCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAACTTTTCAGGCGAA9470.1499530506024249No Hit
GTATAAGGTGATCGCAGGTTGTGCAATCATTGCTCAAAAGGGTGTACACC8740.13839383973233302No Hit
CAATTGATATTAGATATTTTTGCAAAGCGGGCGAGAACGAGAGAAGGCAA8350.13221837091132502No Hit
GACCATAATGTGATCCCTTCCGGCGGTCGGTATAAAATCAGGCAGTTTTT8150.12905146382362861No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA7330.11606714476407333No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7290.11543376334653406No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC7260.11495872728337961No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC7020.1111584387781439No Hit
ATCTAATTCACCTCCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAA6910.10941663987991089No Hit
GTTAATAATTCTGGCAATTCGTCTCCACACTAGAAGTCGACGAACAACGA6810.10783318633606268No Hit
TTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCC6550.10371620712205736No Hit
GTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGG6540.10355786176767252No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT6500.10292448035013324No Hit
GTGTAGGACTCGTCGCATTGGATGACGATGCCTAGTACTGTGCGCCAATT6370.10086599074313057No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA17950.053.2781871
CAACGCA23050.039.7633675
ATCAACG23800.038.115343
GGTATCA7800.038.0508041
TAACCGT757.4357413E-637.6018686
AACGCAG26750.035.4933556
TATCAAC27550.034.8039052
ACGCAGA27050.033.8807037
TCAACGC27750.032.859294
CGCAGAG27750.032.6873258
GCAGAGT28900.031.5492429
ATAACCG902.5912079E-531.334895
GTACTAG5150.028.3577861
GTACATG27500.027.5810661
TAGGCAT5900.027.0860925
TACATGG28600.026.9523892
AGAGTAC28050.026.47337210-11
AACCGTC1258.018269E-626.3192217
ACATGGG28250.026.121653
TAACCAC1807.479684E-926.1124085
Produced by FastQC (version 0.11.2)