FastQCFastQC Report
Sun 14 Apr 2019
SRR6575945_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575945_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences579505
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA12310.21242267107272586No Hit
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC11200.1932683928525207No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT10110.1744592367624093No Hit
GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC9340.1611720347537985No Hit
CCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAACTTTTCAGGCGAA8630.14892019913546908No Hit
GTATAAGGTGATCGCAGGTTGTGCAATCATTGCTCAAAAGGGTGTACACC8110.1399470237530306No Hit
GACCATAATGTGATCCCTTCCGGCGGTCGGTATAAAATCAGGCAGTTTTT8090.1396019016229368No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC7900.13632324138704582No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC7850.13546043606181135No Hit
CAATTGATATTAGATATTTTTGCAAAGCGGGCGAGAACGAGAGAAGGCAA7790.13442506967153003No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA6800.11734152423188757No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6640.11458054719113725No Hit
GTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGG6630.11440798612609036No Hit
GTGTAGGACTCGTCGCATTGGATGACGATGCCTAGTACTGTGCGCCAATT6620.11423542506104346No Hit
GTTAATAATTCTGGCAATTCGTCTCCACACTAGAAGTCGACGAACAACGA6490.11199213121543387No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT6470.11164700908534009No Hit
TTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCC6460.11147444802029319No Hit
ATCTAATTCACCTCCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAA6360.10974883736982424No Hit
ATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCTGGGGAGATTA5860.10112078411747957No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT5830.10060310092233889No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA17100.058.1291121
ATCAACG22550.042.9279373
CAACGCA22700.042.6442685
AACGCAG25100.038.1922956
GGGTACC2600.037.9546747
ACGCAGA25400.037.5562027
TATCAAC26750.037.245082
CGCAGAG26050.036.463888
GGTATCA6450.035.788521
TCAACGC27900.034.8646624
CATGGGT4800.034.2646374
GCATTAG550.002685119634.2613681
ATAGGTA2400.033.31151694
GCAGAGT28850.033.250919
TGGGTAC3000.032.894056
ATGGGTA3550.031.76895
CTACTAA600.00414893131.3493069
GTACTAG4000.030.6210941
TAACCAC1701.2187229E-1030.4062636
CGGGCGT2950.030.2657156
Produced by FastQC (version 0.11.2)