FastQCFastQC Report
Sun 14 Apr 2019
SRR6575946_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575946_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences984980
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA16060.16304899591869887No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC13320.13523117220654227No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA10790.10954537147962395No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA10330.10487522589291154No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG9850.10000203049808118No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA16900.064.254891
GGTATCA4800.050.9264031
TCAACGC23900.045.2295884
ATCAACG24700.043.9549453
CAACGCA24800.043.7777065
AACGCAG25350.043.38416
TATCAAC28250.038.605612
TAGGTAT3400.035.940755
ACGCAGA30800.035.2495847
TTAGGTA3650.033.4790534
CGCAGAG33150.032.750748
GTACATG43100.031.6301771
GTCTTAG4850.031.0162371
TACATGG44150.030.4520262
GCAGAGT35650.030.322229
ACATGGG43800.030.0453053
AGGTATA4150.029.4454336
ACCTAAG7950.027.7914771
CTTAGGT4350.027.0111713
AGAGTAC31550.026.88874410-11
Produced by FastQC (version 0.11.2)