FastQCFastQC Report
Sun 14 Apr 2019
SRR6575948_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575948_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences520058
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG9660.18574851266589498No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6590.12671663545219958No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA5490.10556514850266702No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGTAT353.6366018E-667.140258
GTATCAA13750.047.8618931
TACCGTA450.001013636841.7761547
ATCAACG16900.038.377213
TCAACGC16900.038.377214
CAACGCA17350.037.6527185
CCGTATG500.001701495437.5985379
AACGCAG17350.037.3818326
GTACATG29700.034.3453371
GTACCGT702.183862E-433.5701266
TACATGG30500.033.290352
ACATGGG30750.032.7076723
TATCAAC20600.032.4030272
ACGCAGA20400.031.7928817
CGCAGAG20500.031.6377948
TACTATA1351.3222234E-831.3381422
CATGGGT6950.028.4017734
CATGGGA15400.028.076834
GCAGAGT24450.026.911029
GGTATCA6050.026.4172741
Produced by FastQC (version 0.11.2)